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Title: A procedure for isolating vacuoles from leaves of the halophyte Suaeda maritima / M.N.H. Dracup and H. Greenway.
Author: Greenway, H. ; Dracup, M. N. H.
Year: 1985.
Series: Plant, cell and environment, v. 8.
Description: p. 149-154 ; 30 cm.
Subject: Vacuoles. ; Salt tolerance. ; Salinity. ; Protoplasts. ; Suaeda maritima. ; Halophytes.
Notes: Reprint : Plant, cell and environment, v. 8, p. 149-154, 1985. 2 tables, 3 figures.
24 ref.
Type: Pamphlet
Abstract: A method is described for isolating protoplasts and vacuoles from leaves of the halophyte Suaeda maritima. Integrity of the protoplasts and vacuoles was tested by staining and shown to be more than 75 per cent, while use of biochemical markers, staining and light microscopy suggested a high degree of purity of the vacuoles. Phosphatase and NADH cytochrome-c-reductase were associated with vacuoles; phosphatase showed an eight-fold enrichment and NADH cytochrome-c-reductase a 3.5-fold enrichment relative
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