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Title: Determination of free space, growth, solute concentrations and parameters of water relations of suspension-cultured tobacco cells / M. Dracup... [et al]
Author: Dracup, M. N. H.
Year: 1986.
Series: Plant, cell and environment, v. 9.
Description: p. 693-701 ; 28 cm.
Subject: Tobacco. ; Growth. ; Solutions. ; Culture techniques. ; Plant water relations.
Notes: Reprint : Plant, cell and environment v. 9, p. 693-701, 1986. 1 table, 3 figures.
48 ref.
Type: Pamphlet
Abstract: Methods were developed for measuring water content of the free space of suspension-cultured tobacco cells using 3H- or 14C-sorbitol. Sorbitol was not taken up by cells in significant quantities over the 3 min taken to label free space. Free space accounted for 50-60 per cent of the water content of cell pellets. Measurement of free space is necessary for measuring growth on a fresh or dry weight basis, solute concentrations and parameters of water relations of cells. Techniques for making these measur
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