Abstract:
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The first stage of the project examined the species specific osmotic characteristics of sheep and cattle embryos. The results revealed that sheep and cattle embryos are more permeable to ethylene glycol than to glycerol. The practical implication of this finding is that sheep and cattle embryos cryopreserved in ethylene glycol are less likely to suffer osmotic damage during cryoprotectant removal than those cryopreserved in glycerol. The second stage of the project examined the effects of the compositi ces the time required for the cooling process. Based on these advantages and on results reported, rapid cryopreservation can now be recommended for commercial use.
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